超高效液相色谱法测定烟叶中的茄尼醇含量

张婕; 汪云松; 蔡凯; 向章敏; 周淑平; 许冬青; 耿召良

doi:10.3969/j.issn.1007-5119.2012.06.016

超高效液相色谱法测定烟叶中的茄尼醇含量

1.
贵州省烟草科学研究所,贵阳 550081
2.
云南大学,昆明 650091

基金项目: 贵州省烟草公司重点科技计划项目(2009-09)

详细信息

作者简介:
张婕,女,实验师,主要从事烟草化学研究工作。E-mail:zj0851@126.com。

通讯作者:
耿召良

中图分类号: S572.03
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出版历程
- 收稿日期: 2011-12-08
- 修回日期: 2012-05-02
- 刊出日期: 2012-12-29

Determination of Solanesol Content in Tobacco Leaves by Using Ultra-high Pressure Liquid Chromatography

1.
Guizhou Tobacco Research Institute, Guiyang 550081, China
2.
Yunnan University, Kunming 650091, China

摘要

摘要: 为了更简便高效地分析烟叶中游离茄尼醇的含量,建立了一种超高效液相色谱(UPLC)快速分析方法。将烟叶样品用甲醇超声提取10 min,经膜过滤直接进行超高效液相色谱分析;在1.7 μm BEH C18色谱柱上用甲醇洗脱,在5 min内实现了茄尼醇的快速分离,并用电喷雾四级杆-飞行时间质谱(Q-TOF-MS)对m/z 613.57和631.58进行了定性分析。结果表明,茄尼醇的质量浓度范围为0.765～76.5 μg/mL时,峰面积与质量浓度具有良好的线性关系,相关系数为0.99924,方法的检出限7.65 ng/mL,RSD为2.62%,平均回收率为91.1%。该方法适合于烟叶中游离茄尼醇含量的快速准确测定。
- 茄尼醇 /
- 烟叶 /
- 超高效液相色谱 /
- 四级杆-飞行时间质谱
Abstract: In order to determine the content of free solanesol in tobacco leaves quickly and efficiently, a rapid Ultra-high Pressure Liquid Chromatography (UPLC) method was developed. By ultrasonic extraction with methanol, the extracting solution was membrane filtered to analyze by UPLC. The extract was eluted with methanol onto a short 1.7 μm BEH C18 column, and solanesol was separated rapidly in 5 min. According to the ion peaks of m/z 613.57 and 631.58, qualitative analysis was carried out by Electrospray Ionization-Quadropole Time-of-Flight Mass Spectrometry (Q-TOF-MS). The results showed that the linearity range of the proposed method was 0.765-76.5 μg/mL, the RSD% was 2.62%, and the average recovery rate was 91.1%. The method was appropriate for quick and sensitive determination of free solanesol in tobacco.
- solanesol /
- tobacco leaf /
- UPLC /
- Q-TOF-MS

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参考文献(15)

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