Abstract: To investigate the resource optimization configuration mode of tobacco production, this study established an econometric model by input-output index of the tobacco production. The relationship between the tobacco input and output was investigated by using production data from Enshi in Hubei Province. The result showed that in the tobacco production process, planting acreage, fertilization input, and fuel input had the greatest effect on production efficiency. planting in larger acreage, rational fertilization, implementation of the mechanized operations could lead to higher efficiency of tobacco production.