Abstract: In order to identify the soil factors of shandong tobacco areas on the effect of oil in flue-cured tobacco leaves, the relationship between land conditions (landforms, soil types, soil textures, elevations and soil nutrient conditions) of four ecotypes in Shandong and oil in different parts of flue-cured tobacco leaves was analyzed. The result showed that different landforms and soil textures played important roles in the oil in middle leaves and upper leaves, especially for the middle leaves. The upper and middle leaves from hilly areas was significantly better than those from mountain areas. The oil in tobacco leaves from argillaceous soil performed worse on the whole. Oil in middle leaves was significant lower than those from sandy loam. There was no significant difference between neutral loam and sandy loam soil. Soil types, elevations and soil nutrient conditions had no significant effect on oil in different parts of tobacco leaves. There was no significant difference about oil in tobacco leaves between the four ecotypes.