Abstract: Floating pool system for screening bacterial wilt resistance of tobacco seedlings was developed by building temperature controlled water heating float pool and inoculating with pool water in greenhouse. The resistance result of seven known tobacco varieties evaluated by floating pool water inoculation method was consistent with the varieties inherent resistance levels. The advantages of floating pool temperature controlled water inoculation method are rapid disease development, small space needed, less soil-borne disease disturbance, and high result repetition.