Abstract: In order to further explore the chemical component basis of quality characteristics of different position in flue-cured tobacco leaves, the different positions of B2F, C3F and X2F of K326, Yunyan87 and Hongda were divided into leaf tips, middle and base by method of close composition and the biggest bending degree, their distribution characteristics of their lutein and β-carotene were investigated. The results showed that their distribution characteristics were different among varieties and parts. (1) Hongda upper and Cutters: leaf middle>leaf base>l leaf tip, lower: leaf tip>l leaf middle>l leaf base. (2) K326 upper and lower: leaf tip>l leaf base>l leaf middle, Cutters: leaf middle>l leaf tip>l leaf base. (3) Yunyan 87 upper and Cutters: leaf middle>l leaf tip>l leaf base, Lower: leaf middle>l leaf base>l leaf tip. These results provide the basis for slitting, processing and application of the different positions in flue-cured tobacco. These results further explain the reason of different quality characteristics of leaf tips, middle and base and will provide basis for scientifically and rationally making project of cutting, processing and applying these sections.