烤烟CMV抗性基因QTL定位
QTL Analysis of the Resistance Gene to CMV in Flue-cured Tobacco
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摘要: 利用SSR 技术,在抗CMV 烟草中发掘与抗病基因紧密连锁的分子标记,目的是为抗病基因的定位克隆以及培育抗病新品种奠定基础。本研究以抗CMV 的烤烟品种台烟8 号、感病品种NC82 为亲本,构建BC1 代分离群体,对其进行抗性遗传分析。对分布于烟草24 条连锁群上的2317 个SSR 位点进行了多态性筛选,获得了62 个稳定多态性标记,利用这些标记对288 株BC1群体分型数据,用WinQTLCart2.5 进行分析,找到一个与抗性相关的QTL,LOD 值为2.6。研究结论表明,台烟8 号对CMV 的抗性由多基因控制,标记53735 与抗性QTL 紧密连锁,遗传距离为0.1 cM,该QTL 位于10 号连锁群,贡献率为13%。Abstract: In order to obtian gene closely linked with CMV and cultivate resistant varieties, BC1 segregating population was generated from Taiyan-8 (resistant) and NC82 (susceptible) as parents. The BC1 population was used for virus artificial inoculation. 167 polymorphism markers were selected from 2317 SSR markers distributed in 24 tobacco linkage populations and then 62 SSR markers with stability and significant polymorphism were obtained. A tobacco genetic linkage map was constructed according to virus test results of 288 BC1. A QTL related to resistance with LOD equal to 2.6 was obtianed through WinQTLCart2.5 analysis. Our results showed that multiple genes caused the resistance of Taiyan-8; marker 53735 was closely linked to resistance QTL with the genetic distance of 0.1 cM, which could explain 13% of the variation.
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