Abstract: In order to obtian gene closely linked with CMV and cultivate resistant varieties, BC1 segregating population was generated from Taiyan-8 (resistant) and NC82 (susceptible) as parents. The BC1 population was used for virus artificial inoculation. 167 polymorphism markers were selected from 2317 SSR markers distributed in 24 tobacco linkage populations and then 62 SSR markers with stability and significant polymorphism were obtained. A tobacco genetic linkage map was constructed according to virus test results of 288 BC1. A QTL related to resistance with LOD equal to 2.6 was obtianed through WinQTLCart2.5 analysis. Our results showed that multiple genes caused the resistance of Taiyan-8; marker 53735 was closely linked to resistance QTL with the genetic distance of 0.1 cM, which could explain 13% of the variation.