高效液相色谱法同时测定植烟土壤和水中3种除草剂残留量

丁春霞; 龚道新; 肖浩; 任颖俊; 杨丽华

doi:10.3969/j.issn.1007-5119.2013.05.019

高效液相色谱法同时测定植烟土壤和水中3种除草剂残留量

丁春霞^1,2,3,
龚道新^2,3,,
肖浩²,
任颖俊²,
杨丽华²

1.
湖南农业大学理学院, 长沙 410128
2.
湖南农业大学资源环境学院, 长沙 410128
3.
湖南农业大学烟草研究院, 长沙 410128

详细信息

作者简介:
丁春霞,女,讲师,在读博士研究生,主要研究方向为农药残留分析检测。E-mail:dcx@hunau.net。

通讯作者:
龚道新

中图分类号: S572.01;TS411
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出版历程
- 刊出日期: 2013-10-29

Simultaneous Determination of Three Kinds of Herbicide Residues in Soil and Water in Tobacco Planting Field

1.
College of Science, Hunan Agricultural University, Changsha 410128, China
2.
College of Resource and Environment, Hunan Agricultural University, Changsha 410128, China
3.
Tobacco Research Institute, Hunan Agricultural University, Changsha 410128

摘要

摘要: 为了评价植烟土壤中常用3种除草剂对生态环境的安全性,建立了同时测定植烟土壤和水中高效氟吡甲禾灵、精吡氟禾草灵和精喹禾灵3种除草剂残留量的高效液相色谱分析方法。水中3种农药残留用二氯甲烷-丙酮(20:1,v:v)混合液提取,土壤中农药残留用丙酮提取,经弗罗里硅土净化后,采用HPLC法外标法定量。结果表明,3种除草剂在0.0050～5.0 mg/L范围内线性良好,相关系数均在0.99以上;土壤中添加水平在0.050～1.0 mg/kg时,3种除草剂在土壤中平均添加回收率为77.5%～110.3%,相对偏差为1.6%～10.2%,当水中添加水平在0.0050～1.0 mg/L时,回收率为88.9%～101.2%,相对偏差为0.9%～8.2%,符合农药残留分析方法要求。
- 高效氟吡甲禾灵 /
- 精吡氟禾草灵 /
- 精喹禾灵 /
- 高效液相色谱 /
- 残留
Abstract: In order to evaluate the bio-environmental safety of three kinds of herbicides, which were often applied to tobacco soil, an analytical method was established to simultaneously determine the residues of haloxyfop-p-methyl, fluazifop-p-butyl and quizafop-p-ethyl in soil and water in tobacco planting field by high performance liquid chromatography. The residues in water was extracted with mixed solution of methylene chloride-acetone (20:1, v:v). The soil was extracted with acetone and the extract was cleaned up by a Florisil chromatography column. The quantification was performed by external standard. The linearity was satisfied (r>0.99) in concentration range of 0.0050-5.0 mg/L. The average recoveries of this three herbicides varied from 77.5% to 110.3% with relative standard deviation of 1.6%-10.2%, when three kinds of herbicides were spiked at 0.050-1.0 mg/kg level in soil. The average recoveries of the three herbicides varied from 88.9% to 101.2%, with relative standard deviation of 0.9%-8.2%, when three kinds of herbicides were spiked at 0.0050-1.0 mg/L level in water. The method in this study could meet the requirements of pesticide residue analysis criteria.
- haloxyfop-p-methyl /
- fluazifop-p-butyl /
- quizafop-p-ethyl /
- high performance liquid chromatography /
- residue

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参考文献(17)

[1]	Sakamoto M, Tsutsumi T. Applicability of head space solid-phase micro-extraction to the determination of multi-class pesticides in waters[J]. Journal of Chromatography A, 2004, 1028(1): 63-67.
[2]	韩熹莱. 中国农业百科全书(农药卷)[M]. 北京:农业出版社,1993:164-165.
[3]	孙亚林,朱文达,陈文,等. 精喹禾灵对夏大豆田间的控草效果和光照及养分的影响[J]. 华中农业大学学报,2009,28(2):161-163.
[4]	Mustfa Y, Arikan E S. Genotoxicity testing of quizalofop-p-ethyl herbicide using the Allium cepa anaphase-telophase chromosome aberration assay[J]. Caryologia, 2008, 61: 45-52.
[5]	Elefsiniotis I S, Liatsos G D, Stamelakis D, et al. Case report: mixed cholestatic/hepatocellular liver injury induced by the herbicide quizalofop-p-ethyl[J]. Environ. Health Perspect, 2007, 115: 1479-1481.
[6]	Borjan M. Evidence on developmental and reproductive toxicity of quizalofop-ethyl[R]. California: California Environmental Protection Agency (CEPA), 1999.
[7]	Doganlar Z B. Quizalofop-p-ethyl-induced phytotoxicity and genotoxicity in Lemna minor and Lemna gibba[J]. Journal of Environmental Science and Health, 2012, 47(11): 1631-1643.
[8]	Zhang X, Wang S, Wang Y, et al. Differential enantioselectivity of quizalofop-ethyl and its acidic metabolite:Direct enantiomeric separation and assessment of multiple toxicological endpoints[J]. Journal of Hazardous Materials, 2011, 186: 876-882.
[9]	俞少勇,陈日萍,陈彤,等. 高效氟吡甲禾灵对大鼠睾丸生精细胞的损伤作用[J]. 毒理学杂质,2006,20(6):390-392.
[10]	曾得意,施海燕,李波,等. 气相色谱法(NPD)测定水和土壤中精喹禾灵的残留量[J]. 贵州大学学报:自然科学版,2006,23(2):184-187.
[11]	苏建峰,钟茂生,张光军,等. 固相萃取-气相色谱-质谱联用测定葱属蔬菜中二甲嘧菌胺和吡氟禾草灵残留量[J]. 应用化学,2010,27(5):590-593.
[12]	田华,王延华. 10.8%精喹禾灵水乳剂的高效液相色谱分析[J]. 应用化工,2011,40(7):1291-1289.
[13]	谢勇波,周清明,龚道新,等. 高效液相色谱法测定土壤、烟草和油菜中高效氟吡甲禾灵残留[J]. 农药,2011,50(3):209-212.
[14]	李桂红,张东飞,赵瑞,等. 超高效液相色谱串联质谱测定大豆和土壤中精喹禾灵的残留分析方法[J]. 农药科学与管理,2011,32(8):37-40.
[15]	刘慧,曹赵云,张利强,等. 分散固相萃取-液相色谱-串联质谱法测定农产品中吡氟甲禾灵[J]. 分析实验室,2011,30(3):83-86.
[16]	Zeng D Y, Shi H Y, Li B, et al. Development of an enzyme-linked immunosorbent assay for quantitative determination of quizalofop-p-ethyl[J].Agricultural and Food Chemistry, 2006, 54: 8682-8687.
[17]	Bao H, Fang S, Liu Z, et al. Development of an enzyme-linked immunosorbent assay for the rapid detection of haloxyfop-p-methyl[J]. Journal of Agricultural and Food Chemistry , 2010, 58(14): 8167-8170.