Abstract: In order to evaluate the yield ability, stability, and eco-distribution of tobacco cultivars effectively, genotype main effect and genotype-environment interaction (GGE) biplot was introduced as a new and powerful approach to analyze the leaf yields of 11 flue-cured tobacco (cultivars or lines) tested at 7 planting areas of middle flavor type. The results showed that the GGE biplot was highly representative, which explained 71.9% of the total variation of production value and 64.4% of the total yield variation. The cultivars with highest yields and of most stability and most adaptability were "Bina 1" and "Guiyan 1". The 7 test sites were broadly classified into 3 environments, as region one including Zunyi, Wulong, and Sangzhi, region two including Xunyang, and Mengyin, region three including Guiding and Xianfeng, in which the best genotypes were "Bina 1","Qinyan 98", and "HB032" respectively. The result of some genotypes specifically adapted to certain environments is useful for special cultivars screening.