Abstract: In this paper, screening of plants inhibiting Tobacco Mosaic Virus (TMV) activity was achieved under the disease measuring standard with half leaf lesion experiment. Botanical extracts were obtained from 24 kinds of plants, including Artemisia Argyi, Radix Isatis, Bupleurum, etc, and their inhibiting effect on TMV activity were tested by passive activation of TMV in vitro and inhibiting TMV primary infection. Pot experiments were used to investigate the protection and therapeutic effect of virus. The results indicated that the abstracts from ginkgo, gardenia, phytolacca and red peony root had great inhibiting effect on TMV, which is promising for further development and utilization.