拮抗细菌对烟草花叶病毒（TMV）的抑制作用研究

A Study on Resistance of Antagonistic Bacteria to TMV

摘要: 从发生烟草花叶病毒（TMV）的烟田耕作层土样中分离到对TMV有拮抗作用的菌株By33和By88。生物测定试验表明，By33和By88的无菌发酵液对TMV的体外抑制作用分别为81.81%和83.29%，涂抹菌液24 h后接种TMV，仍有73.01%和66.98%的预防作用。硫酸铵沉淀无菌发酵液、PBS透析获得拮抗蛋白；拮抗蛋白对TMV的体外抑制作用分别为85.38%和87.47%，并且在274.00 nm处有明显的蛋白吸收峰，为典型的蛋白质吸收光谱。此外，通过叶圆片法和透析法生物测定表明，抗病毒蛋白与TMV粒子结合作用为不可逆，有抑制TMV在烟株体内复制的作用。

Abstract: The antagonistic bacteria strains By33 and By88 were isolated from top soils of tobacco planting fields. The results showed that the invitro inhibition of sterile fermented broths of By33 and By88 to TMV was 81.81% and 83.29% respectively, and 73.01% and 66.98% respectively inoculating TMV 24h after smearing fermented broths. The antagonistic proteins obtained by ammonium sulfate deposition had the control of 85.38% and 87.47% to TMV, with absorption apexes in 274 nm. The study also showed that the combination of antagonistic proteins and TMV was inreversible, and could restrain the copy of TMV in tobacco.