Abstract: In this paper, authors investigated the effects of composted barnyard manure on tobacco bacterial wilt in flue-cured tobacco-succession-planting on yellow upland soils and yellow limy soils, and in flue-cured tobacco-rotation-planting on yellow upland soils by field experiments. The results showed that variety resistance was the main factor that resulted in the difference among the treatments. Resistance of tobacco variety K346 was significantly superior to that of K326. Light epidemic of tobacco bacterial wilt in flue-cured tobacco-rotation-planting on yellow upland soils among three field types indicated that rotation was effective measures for controlling this disease. Soils of composted barnyard manure had some effects of inhibition, which can postpone beginning date and decreased epidemic degree of this disease.