fps 转基因烤烟类胡萝卜素及其降解产物的研究
Studies on Cartenoids and Aromatic Components of the Transgenic Flue- cured Tobacco Expressing fps Gene
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摘要: 为探索法呢基焦磷酸合酶( fps) 对烤烟中类胡萝卜素及其降解产物的影响, 利用常规和GS / MS 方法, 对转fps 基因烟草株系( K- 4、K- 6、K- 17、K- 35) 烤后烟叶中类胡萝卜素含量及萜烯类香气物质含量进行了分析, 结果发现, 烤后烟叶类胡萝卜素含量有较大差异, 与未转基因对照相比普遍有所提高, 其中K- 35 含量最高; 8 种类胡萝卜素降解产物及茄酮、新植二烯含量有不同程度的提高, 其中K- 35 香气降解产物含量整体较高, 表明外源fps 基因在烟草中的表达对类胡萝卜素合成具有促进作用, 从而有利于烟叶品质的提高。Abstract: In order to explore the effect of farnesyl pyrophosphate synthase gene (fps) on the flue- cured tobacco, cartenoids and aromatic components of transgenic tobacco plant lines(K- 4、K- 6、K- 17、K- 35) expressing fps gene of Mentha spicata L. were studied.Cartenoids content and the terpene aromatic components of flue- cured tobacco leaves were determined by GS/MS. Cartenoids content of transgenic tobacco leaves increased greatly compared to that of non- transgenic tobacco plants. The differences also existed among different transgenic lines, and the cartenoids contents of K- 35 lines is the highest . 8 kinds of aromatic components degraded from cartenoids were detected, and the content increased compared to the control. Solanone and neophytadiene were also increased in transgenic lines. This results showed that the expression of foreign fps gene in tobacco could improve the aromatic quality of tobacco leaves by enhancing terpene metabolites.
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