Abstract: The experiment conducted with flue - cured tobacco variety NC82 revealed that the effects of clipping in seedling stage on growth and development of tobacco could undergo different changes with temperature, more significant under low temperature than high temperature. For tobacco plants with premature flower resulted from lower temperature, the flower budding stage could be postponed over 20 days by clipping treatment, and the effect was furthered with added clipping. Clipping could reduce the plant height in 50 days after transplanting, but no effect on final height. The total leaf number of clipped plant was one more than nonclipped plant, but clipping didn’t affect the microstructure of flower bud differentiation significantly.