Abstract: The soil medium element and microelement contents in material base of Hongyun Tobacco Group were studied in this study. The results showed that among 23 raw material base counties, Tengchong exhibited the available B content of 0.52 mg/kg, little higher than the critical value of 0.50 mg/kg, while the other 22 counties were lacking in B or potentially lacking in B. Furthermore, 89.13% of the tobacco planting soil lacked B or potentially lacked B, so additional supplyment of B fertilizer was required. Secondly, except for B, the contents of the other medium elements and microelements were abundant, but some regions belonged to lacking or potentially lacking area, where additional application of medium elements and microelements was needed. Thirdly, the conditions of medium elements and microelements in 23 material base counties could be classified into 5 types by systematic cluster analysis.