一株烟草花叶病毒生防细菌的鉴定及代谢物活性研究

Identification and Antivirus Activity of Bacteria Strain By33 Against Tobacco Mosaic Virus

摘要: 从烟田土样中分离到1 株对TMV 有拮抗作用的细菌By33，通过形态观察、生理生化试验及16S rDNA 同源性序列分析，鉴定该菌为解淀粉芽孢杆菌。通过对By33 代谢产物对TMV 抑制作用测定发现，其代谢物质对温度敏感，80℃处理30 min，丧失大部分活性，遇酸沉淀，碱性条件下稳定，对蛋白酶不敏感。

Abstract: An antagonistic bacteria strain By33 against TMV was isolated from tobacco soils. Basing on morphological observation, physiological & biochemical test and 16S rDNA sequence analysis, By33 was identified as Bacillus amyloliquefaciens. The active substance lost most active control at 80 ℃, and was unstable in acid condition yet stable in neutral or slight alkaline condition, with resistant to proteolytic enzymes.