Abstract: China has the world largest tobacco germplasm resource genebank. As the basic foundation of breeding project and sustainable development of the tobacco industry, Chinese State Tobacco Monopoly Bureau attaches importance to germplasm resources study and the major projects, constriction of tobacco germplasm resources platform, was formally implemented in 2007. In this paper, the background of this major project was reviewed, and the significance of the project was summarized comprehensively. We then introduced the content of platform construction from 4 areas, including collection and conservation, characterization and evaluation, resources sharing as well as germplasm enhancement. Finally, some great progress was summarized. Finally, we discussed the directions in the future.