茉莉酸甲酯对烟草α-淀粉酶抑制剂的诱导作用

Inducement Effect of Methyl Jasmonate on Tobacco α-amylase Inhibitor

摘要: 研究了不同浓度的茉莉酸甲酯（MeJA）对烟草α-淀粉酶抑制剂诱导的时间及浓度效应。在斜纹夜蛾取食不同浓度的MeJA 诱抗处理的烟草叶片1～3 d 后，对中肠α-淀粉酶活性进行了检测。结果显示，0.01，0.1，1.0，4.0 mmol/L 和10.0mmol/L 5 种浓度的MeJA 处理烟草后，烟叶α-淀粉酶抑制剂含量明显增加，而4.0 mmo1/L MeJA 诱抗处理48 h 时诱导烟草产生的α-淀粉酶抑制剂活性最强。离体检测结果表明，斜纹夜蛾取食MeJA 处理的烟草叶片后，中肠α-淀粉酶活性降低。

Abstract: The time and concentration inducement effects of methyl jasmonate (MeJA) on а-amylase inhibitor in tobacco leaves were studied, and the activities of Spodoptera litura (F.) midgut α-amylase was measured, which feed on the tobacco leaves after 1-3d MeJA spraying. The results showed that, after treated with five concentrations of MeJA (0.01, 0.1, 1.0, 4.0, 10.0 mmol/L), the content of α-amylase inhibitor activity in leaf increased significantly. The 4 mmol/L MeJA induced the strongest response of а-amylase inhibitor activity in tobacco leaves after 48 h treatment. In vitro assay demonstrated that the Spodoptera litura (F.) midgut а-amylase activity decreased after feed on the treated tobacco leaves.