甲氨基阿维菌素苯甲酸盐在烟草及土壤中残留分析方法的研究

Residue Analysis of Emamectin Benzoate in Tobacco and Soil

摘要: 采用反相高效液相色谱法对烟草及土壤中甲氨基阿维菌素苯甲酸盐emamectin benzoate 进行残留量分析。样品采用丙酮/水提取，Florisil-SPE 柱净化，紫外检测器（UVD）测定。甲氨基阿维菌素苯甲酸盐在本方法仪器条件下最低检出限为2.0×10^-10 g（B1a）；1.0×10^-9 g（B1b）。样品在烟叶中的平均回收率为94.92%~102.18%，相对标准偏差为2.66%~3.69%；在土壤中的平均回收率为96.76%~99.02%，相对标准偏差为2.23%~4.48%。

Abstract: Residue analysis of emamectin benzoate in tobacco and soil by Reversed-Phase high performance liquid chromatography was assessed. Residues were first extracted with acetone and water, filtrated with Florisil-SPE columns, and then determined with UV detection. Lowest detection limit with this method were 2.0×10^-10g（B1a）and 1.0×10^-9g（B1b）. The average spiked recoveries were 94.92%-102.18% in tobacco leaves, and 96.76%-99.02% in the soil. The precision of the method is acceptable with relative standard deviation (RSD) of 2.66%-3.69% for tobacco leaves and 2.23%-4.48% for the soil, respectively.