中烟特香301特异SNP指纹图谱构建及应用

田震; 孙晋浩; 武钰存; 蒋龙; 赵勇; 冯慧馨; 任民; 孙治峰; 付秋娟; 宗浩; 刘文涛; 刘贯山; 吴新儒; 李少鹏

中烟特香301特异SNP指纹图谱构建及应用

Construction and Application of Specific Fingerprint of Flue-cured Tobacco Variety Zhongyantexiang 301

摘要

摘要: 指纹图谱可以用于品种真实性鉴定，为品种的推广种植和工业利用提供技术保障。本研究采用全基因组重测序技术，对包括烤烟新品种中烟特香301在内的33份烟草种质资源进行了单核苷酸多态性（SNP）变异分析，从中鉴定获得中烟特香301特异SNP位点268个。利用包括烤烟、雪茄烟、香料烟、白肋烟、晒烟在内的62份不同类型烟草种质资源进行了筛选，初步获得有效SNP标记21个。以2022—2023年山东产区的中烟特香301及其他5个主栽品种的大田新鲜烟叶和初烤烟叶为材料，利用上述21个SNP标记进行品种真实性检测，结果显示，18个SNP标记在所有烟叶中可全部检测到中烟特香301特异SNP位点。利用这18个SNP标记构建的指纹图谱可以用于中烟特香301品种真实性鉴定。

Abstract: Fingerprinting is an effective method for verifying the authenticity of plant varieties, providing crucial technical support for the expansion of cultivation and the industrial application of these varieties. In this study, single nucleotide polymorphism(SNP) variations were analyzed across 33 tobacco germplasm resources, including the new flue-cured tobacco variety Zhongyantexiang 301, taking advantage of the whole genome resequencing technology. This analysis identified a total of 268 specific SNP loci unique to Zhongyantexiang 301. After screening 62 tobacco germplasm resources from various market types—flue-cured, cigar, oriental, burley, and sun-cured tobacco—21 effective SNP markers were preliminarily developed. Field-fresh and primary flue-cured leaves of Zhongyantexiang 301, along with five other main cultivars from the Shandong growing area, were collected between 2022 and 2023 for variety authenticity identification using the 21 SNP markers. The results showed that 18 of these markers successfully detected the specific SNP loci of Zhongyantexiang 301 in all tobacco samples, demonstrating their effectiveness in authenticating the variety.

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