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    肖鑫, 丛萍, 徐艳丽, 况帅, 杨如意, 战徊旭, 姜淑祯, 张燕, 董建新. 长期不同施肥制度下植烟土壤氮组分变化及影响因素分析[J]. 中国烟草科学, 2023, 44(6): 12-20. DOI: 10.13496/j.issn.1007-5119.2023.06.003
    引用本文: 肖鑫, 丛萍, 徐艳丽, 况帅, 杨如意, 战徊旭, 姜淑祯, 张燕, 董建新. 长期不同施肥制度下植烟土壤氮组分变化及影响因素分析[J]. 中国烟草科学, 2023, 44(6): 12-20. DOI: 10.13496/j.issn.1007-5119.2023.06.003
    XIAO Xin, CONG Ping, XU Yanli, KUANG Shuai, YANG Ruyi, ZHAN Huaixu, JIANG Shuzhen, ZHANG Yan, DONG Jianxin. Analysis of Changes in Nitrogen Components and Influencing Factors in Tobacco Soil under Different Long-term Fertilization Regimes[J]. CHINESE TOBACCO SCIENCE, 2023, 44(6): 12-20. DOI: 10.13496/j.issn.1007-5119.2023.06.003
    Citation: XIAO Xin, CONG Ping, XU Yanli, KUANG Shuai, YANG Ruyi, ZHAN Huaixu, JIANG Shuzhen, ZHANG Yan, DONG Jianxin. Analysis of Changes in Nitrogen Components and Influencing Factors in Tobacco Soil under Different Long-term Fertilization Regimes[J]. CHINESE TOBACCO SCIENCE, 2023, 44(6): 12-20. DOI: 10.13496/j.issn.1007-5119.2023.06.003

    长期不同施肥制度下植烟土壤氮组分变化及影响因素分析

    Analysis of Changes in Nitrogen Components and Influencing Factors in Tobacco Soil under Different Long-term Fertilization Regimes

    • 摘要: 探究不同施肥处理土壤氮组分变化及其与土壤化学性质、酶活性和基因丰度之间的关系。试验共设5个处理:不施肥(CK)、无机肥(NPK)、有机肥(OM)、无机肥与有机肥配施(NPKO)和无机肥与冬闲季种植绿肥(NPKG)。结果表明:(1)与CK和NPK相比,OM和NPKO处理显著增加土壤全氮、微生物量氮、可溶性有机氮和硝态氮含量。(2)与CK相比,OM和NPKO处理显著增加土壤中全碳、微生物量碳和有效磷含量;与NPK相比,OM处理对碳组分和有效磷含量提升效果显著。(3)与CK相比,OM、NPKO和NPKG处理显著提高土壤氨单加氧酶、硝酸还原酶及土壤脲酶活性和amoBnirKnirSnosZ功能基因丰度,增幅分别为9.47%~31.13%、46.46%~1613.48%、50.41%~323.72%、108.19%~611.02%、64.19%~252.15%、153.20%~373.21%和56.90%~299.47%,而NPK处理显著降低土壤硝酸还原酶、土壤脲酶及土壤亮氨酸氨基肽酶活性和amoAnirK功能基因丰度。(4)土壤硝酸还原酶、脲酶和氧化亚氮还原酶是土壤氮组分变化的主要影响因子且土壤硝酸还原酶与土壤氮组分之间具有极显著相关性(p<0.01)。在长期施肥条件下,土壤氮转化关键酶活性是影响植烟土壤氮素转化的关键因素。

       

      Abstract: Five treatments were analyzed to investigate the changes in soil N fractions and their relationship with soil chemical properties, enzyme activities and gene abundance in different fertilization treatments, including no fertilization (CK), single application of inorganic fertilizer (NPK), single application of organic fertilizer (OM), combined application of inorganic fertilizer and organic fertilizer (NPKO), and combined application of inorganic fertilizer and winter green manure (NPKG). The results showed that:(1) Compared with CK and NPK, the OM and NPKO treatments significantly increased the contents of soil total nitrogen, microbial biomass nitrogen, water-soluble organic nitrogen and nitrate nitrogen. (2) Compared with CK, the OM and NPKO treatments significantly increased whole carbon, microbial biomass carbon and effective phosphorus contents of the soil; Compared with NPK, the OM treatment had a significant effect on carbon composition and available phosphorus contents. (3) Compared with CK, the OM, NPKO and NPKG treatments significantly increased soil ammonia monooxygenase, nitrate reductase and urease activities and functional gene abundance of amoB, nirK, nirS and nosZ by 9.47%~31.13%, 46.46%~1613.48%, 50.41%~323.72%, 108.19%~611.02%, 64.19%~252.15%, 153.20%~373.21% and 56.90%~299.47%, respectively, while NPK significantly reduced the activities of soil nitrate reductase, urease and leucine aminopeptidase and amoA and nirK functional gene abundance. (4) Soil nitrate reductase, urease and nitrous oxide reductase were the main influencing factors for the change of soil nitrogen composition, and there was a highly significant correlation between soil nitrate reductase and soil N fractions (p<0.01). Under long-term fertilization conditions, the activities of key enzymes related to soil nitrogen conversion was a key factor affecting nitrogen conversion in tobacco-planting soils.

       

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