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    郭光耀, 宋丽云, 江连强, 沈广材, 张富强, 董文凤, 何青云, 申莉莉. 青岛烟区马铃薯Y病毒株系鉴定及系统传导特性[J]. 中国烟草科学, 2023, 44(6): 59-68. DOI: 10.13496/j.issn.1007-5119.2023.06.009
    引用本文: 郭光耀, 宋丽云, 江连强, 沈广材, 张富强, 董文凤, 何青云, 申莉莉. 青岛烟区马铃薯Y病毒株系鉴定及系统传导特性[J]. 中国烟草科学, 2023, 44(6): 59-68. DOI: 10.13496/j.issn.1007-5119.2023.06.009
    GUO Guangyao, SONG Liyun, JIANG Lianqiang, SHEN Guangcai, ZHANG Fuqiang, DONG Wenfeng, HE Qingyun, SHEN Lili. Identification of Potato Virus Y Strains and System Spreading Characteristics in Qingdao Tobacco-growing Areas[J]. CHINESE TOBACCO SCIENCE, 2023, 44(6): 59-68. DOI: 10.13496/j.issn.1007-5119.2023.06.009
    Citation: GUO Guangyao, SONG Liyun, JIANG Lianqiang, SHEN Guangcai, ZHANG Fuqiang, DONG Wenfeng, HE Qingyun, SHEN Lili. Identification of Potato Virus Y Strains and System Spreading Characteristics in Qingdao Tobacco-growing Areas[J]. CHINESE TOBACCO SCIENCE, 2023, 44(6): 59-68. DOI: 10.13496/j.issn.1007-5119.2023.06.009

    青岛烟区马铃薯Y病毒株系鉴定及系统传导特性

    Identification of Potato Virus Y Strains and System Spreading Characteristics in Qingdao Tobacco-growing Areas

    • 摘要: 为明确引起青岛试验田烟草脉坏死及花叶的PVY的发生规律、分子进化和系统传导特性,通过病毒病害表型,发病率和病情指数明确青岛试验田病毒病害流行规律;利用MEGA7构建系统发育树鉴定PVY青岛分离物的株系;通过q RT-PCR,western blotting和PVY-GFP荧光明确PVY侵染烟株的传导路径;通过不同叶位、不同症状病叶取样,利用q RT-PCR检测PVYCPm RNA相对含量,明确显症和隐症叶片的病毒含量差异,以及TMV、CMV、PVY单独、两者混合、三者混合侵染时的干扰或协生作用。结果显示:烟株成熟期,PVY与TMV和CMV混合发生严重,PVY分离物为N:O株系,未突破va基因抗性。在侵染早期,病毒从接种叶沿叶脉进入茎,开始双向运输时,先向根部移动,进而到达苗端;再扩散至上部叶,较慢移动至下部叶;最后伴随烟株生长持续向两端新生部位运输。侵染中期病株上部叶隐症现象显著,病叶症状与病毒浓度呈正相关。烟田中后期,PVY与TMV和CMV混合侵染,病毒间的干扰和协生作用导致症状复杂多变。研究结果有助于提高品种抗性鉴定试验的准确性和药效试验的靶标性。

       

      Abstract: To clarify the occurrence, molecular evolution and system conduction characteristics of potato virus Y, which causes vein necrosis and mosaic leaves in Qingdao experimental field.The prevalence of virus disease in Qingdao experimental field was determined by viral disease phenotype, incidence rate and disease index;MEGA7 was used as a phylogenetic tree to identify the strains of PVY Qingdao isolate;qRT-PCR, western blotting and PVY-GFP were used to clarify the conduction path of PVY-infected plants;diseased leaves were sampled from different leaf positions and disease symptoms, and the relative content of CP mRNA was used to clarify the viral content of leaves with obvious symptoms and latent symptoms;the CP content of the three viruses when TMV, CMV, and PVY infected alone, mixed with the two, and mixed with the three were detected to clarify the interference or synergistic effects of the viruses mixed infection.The results showed that PVY mixed seriously with TMV and CMV infection in the maturity stage, and the PVY isolate was an N:O strain, which did not break through the resistance of the va gene.In the early stage of infection, the virus enters the stem from the inoculated leaf along the veins, and when it begins to transport in two directions, it first moves to the root, and then reaches the seedling tip;then spreads to the upper leaves, and then slowly moves to the lower leaves;finally, it continues to transport to the new parts at both ends as the tobacco plant grows.The recessive symptoms of the upper leaves were obvious in the mid-infection stage, and the symptoms of diseased leaves were positively correlated with the virus concentration.In the middle and late stages of the tobacco field, PVY is mixed with TMV and CMV, and the interference and synergy between viruses lead to complex and changeable symptoms.The research results will help improve the accuracy of the variety resistance identification test and the target of the drug efficacy test.

       

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