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    李舒畅, 李军, 高宪辉, 刘文霖, 常诗瑜, 欧泽洁, 庞雪莉, 孔凡玉. HS-GC-IMS结合ROAV分析烤烟烟叶“糯米香”特征成分[J]. 中国烟草科学, 2023, 44(6): 75-83. DOI: 10.13496/j.issn.1007-5119.2023.06.011
    引用本文: 李舒畅, 李军, 高宪辉, 刘文霖, 常诗瑜, 欧泽洁, 庞雪莉, 孔凡玉. HS-GC-IMS结合ROAV分析烤烟烟叶“糯米香”特征成分[J]. 中国烟草科学, 2023, 44(6): 75-83. DOI: 10.13496/j.issn.1007-5119.2023.06.011
    LI Shuchang, LI Jun, GAO Xianhui, LIU Wenlin, CHANG Shiyu, OU Zejie, PANG Xueli, KONG Fanyu. Analysis of Characteristic “Glutinous Rice”-like Aroma-active Odorants in Flue-cured Tobacco Leaves Based on HS-GC-IMS Combined with ROAV[J]. CHINESE TOBACCO SCIENCE, 2023, 44(6): 75-83. DOI: 10.13496/j.issn.1007-5119.2023.06.011
    Citation: LI Shuchang, LI Jun, GAO Xianhui, LIU Wenlin, CHANG Shiyu, OU Zejie, PANG Xueli, KONG Fanyu. Analysis of Characteristic “Glutinous Rice”-like Aroma-active Odorants in Flue-cured Tobacco Leaves Based on HS-GC-IMS Combined with ROAV[J]. CHINESE TOBACCO SCIENCE, 2023, 44(6): 75-83. DOI: 10.13496/j.issn.1007-5119.2023.06.011

    HS-GC-IMS结合ROAV分析烤烟烟叶“糯米香”特征成分

    Analysis of Characteristic “Glutinous Rice”-like Aroma-active Odorants in Flue-cured Tobacco Leaves Based on HS-GC-IMS Combined with ROAV

    • 摘要: 为准确鉴定烤烟中“糯米香”特征风味化合物,以云南典型“糯米香”(NM)和非“糯米香”(CK)的云烟87烟叶为材料,采用顶空-气相色谱-离子迁移谱(HS-GC-IMS)技术分析其挥发物的组成及相对含量;基于挥发物的相对气味活度值(ROAV)和偏最小二乘法判别分析(OPLS-DA)等统计学方法,鉴定了“糯米香”特征关键标志风味化合物。结果表明,从供试烟叶共鉴定出57种挥发物(包括单体和二聚体),其中2-乙酰基-1-吡咯啉(糯米香),苯乙醛(果香),壬醛(柑橘香)以及2,3-丁二酮(奶甜香)被鉴定为“糯米香”与非“糯米香”烤烟烟叶间的差异性特征风味化合物,其中2-乙酰基-1-吡咯啉是“糯米香”特征的关键贡献化合物,其余3种化合物对烤烟“糯米香”特征香韵具有重要的修饰作用。本研究可能为烤烟“糯米香”特征风味精准调控及评价方法开发提供数据基础。

       

      Abstract: In order to accurately identify the potent odorants in flue-cured tobacco(FCT) leaves with "glutinous rice"-like characteristic, different Yunyan 87 tobacco leaves with or without typical "glutinous rice"-like characteristic from Yunnan province were used as materials in this study.Firstly, volatile compositions and their relative content was analyzed by headspace-gas chromatography-ion migration spectroscopy(HS-GC-IMS).Then, the differential volatile markers between typical "glutinous rice"-like and non-"glutinous rice"-like tobacco leaves samples and the key chemical contributors to the "glutinous rice"-like characteristic were identified based on the relative odor activity value(ROAV) combined with orthogonal partial least squares discriminant analysis(OPLS-DA).A total of 57 common odorants(including monomers and dimers) were identified.2-Acetyl-1-pyrroline("glutinous rice"-like), benzene acetaldehyde(fruity), nonanal(citrus-like), and 2, 3-butanedione(sweet, buttery)are characterized as differential flavor compounds between typical "glutinous rice"-like and non-"glutinous rice"-like tobacco leaves samples, among which 2-acetyl-1-pyrroline is a key contributor to "glutinous rice"-like aroma, the other three compounds play an important role in modifying "glutinous rice"-like characteristic flavor of FCT.The results herein would provide chemical basis for the accurate regulation and the evaluation method development for "glutinous rice"-like characteristic odorants in FCT.

       

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