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    刘琳琳, 芦柯, 胡延奇, 常月勇, 李艳, 时永楠, 李萌, 李晓. 工业发酵雪茄烟叶霉变微生物的分离鉴定及生物学特性研究[J]. 中国烟草科学, 2023, 44(6): 100-105. DOI: 10.13496/j.issn.1007-5119.2023.06.014
    引用本文: 刘琳琳, 芦柯, 胡延奇, 常月勇, 李艳, 时永楠, 李萌, 李晓. 工业发酵雪茄烟叶霉变微生物的分离鉴定及生物学特性研究[J]. 中国烟草科学, 2023, 44(6): 100-105. DOI: 10.13496/j.issn.1007-5119.2023.06.014
    LIU Linlin, LU Ke, HU Yanqi, CHANG Yueyong, LI Yan, SHI Yongnan, LI Meng, LI Xiao. Isolation, Identification and Biological Characterization of Mold Species from Industrial Fermented Cigar Tobacco Leaves[J]. CHINESE TOBACCO SCIENCE, 2023, 44(6): 100-105. DOI: 10.13496/j.issn.1007-5119.2023.06.014
    Citation: LIU Linlin, LU Ke, HU Yanqi, CHANG Yueyong, LI Yan, SHI Yongnan, LI Meng, LI Xiao. Isolation, Identification and Biological Characterization of Mold Species from Industrial Fermented Cigar Tobacco Leaves[J]. CHINESE TOBACCO SCIENCE, 2023, 44(6): 100-105. DOI: 10.13496/j.issn.1007-5119.2023.06.014

    工业发酵雪茄烟叶霉变微生物的分离鉴定及生物学特性研究

    Isolation, Identification and Biological Characterization of Mold Species from Industrial Fermented Cigar Tobacco Leaves

    • 摘要: 为明确引起工业发酵雪茄烟叶霉变的微生物种类及其生物学特性,从工业发酵霉变雪茄烟叶表面分离纯化得到3株霉菌,采用形态学和分子生物学结合的方法进行种属鉴定,并研究其生物学特性。结果表明曲霉属Aspergillus是本研究中引起工业发酵雪茄烟叶霉变的主要菌种,分离纯化得到3株霉变菌株分别为烟曲霉A.tabacinus、黄曲霉A.flavus和蒙地曲霉A.montevidensis。3株霉菌在不同培养基上的生长速度为黄曲霉>烟曲霉>蒙地曲霉。3株霉菌在25~35℃都能生长,40℃只有黄曲霉能生长,45℃及以上3株霉菌均不能生长。黄曲霉生长的最适pH为4,烟曲霉和蒙地曲霉生长最适pH为8~9。3株霉菌均对紫外光较敏感,紫外光照射15 min后致死率均达到90%以上。综上,本研究发现工业发酵过程中引起雪茄烟叶霉变的3种曲霉分别是烟曲霉、黄曲霉和蒙地曲霉,合理运用温度调控和紫外光照射对于防霉控霉有重要意义。

       

      Abstract: To identify the mold species during the industrial fermentation process of cigar tobacco leaves, fungi were isolated and purified from the surface of moldy cigar tobacco leaves, and were identified with the combination of morphological and molecular techniques. The biological characteristics of mold were also investigated. The results showed that the main mold causing moldiness of cigar tobacco leaves were Aspergillus in this study, and three strains of mold were identified as A. tabacinus, A. flavus and A. montevidensis. The growth rate of the three mold strains were in the order of A. flavus > A. tabacinus > A. montevidensis on different culture media. All three mold strains could grow at 25℃ to 35℃, only A. flavus could grow at 40℃, and none of the strains could grow at 45℃or higher temperature. The optimal pH for A. flavus was 4, while A. tabacinus and A. montevidensis were most suitable for pH 8-9. All three mold strains were sensitive to UV radiation, and after 15 minutes of exposure, the mortality rate was over 90%. Overall, the results from this study indicated that the three types of Aspergillus that cause cigar tobacco leaf mold during industrial fermentation are A. tabacinus, A. flavus and A. montevidensis. The rational use of ultraviolet irradiation is important for mold control and prevention.

       

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