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    张怡帆, 李炜, 蒋成勇, 矫海楠, 刘德水, 高丽伟, 张鹏, 任杰, 隋相军, 王洪录, 张士普. 酚氧化酶处理对白肋烟化学成分和烟叶品质的影响[J]. 中国烟草科学, 2024, 45(1): 54-62. DOI: 10.13496/j.issn.1007-5119.2024.01.008
    引用本文: 张怡帆, 李炜, 蒋成勇, 矫海楠, 刘德水, 高丽伟, 张鹏, 任杰, 隋相军, 王洪录, 张士普. 酚氧化酶处理对白肋烟化学成分和烟叶品质的影响[J]. 中国烟草科学, 2024, 45(1): 54-62. DOI: 10.13496/j.issn.1007-5119.2024.01.008
    ZHANG Yifan, LI Wei, JIANG Chengyong, JIAO Hainan, LIU Deshui, GAO Liwei, ZHANG Peng, REN Jie, SUI Xiangjun, WANG Honglu, ZHANG Shipu. Effect of Phenol Oxidase Treatment on Chemical Composition and Leaf Quality of Burley Tobacco[J]. CHINESE TOBACCO SCIENCE, 2024, 45(1): 54-62. DOI: 10.13496/j.issn.1007-5119.2024.01.008
    Citation: ZHANG Yifan, LI Wei, JIANG Chengyong, JIAO Hainan, LIU Deshui, GAO Liwei, ZHANG Peng, REN Jie, SUI Xiangjun, WANG Honglu, ZHANG Shipu. Effect of Phenol Oxidase Treatment on Chemical Composition and Leaf Quality of Burley Tobacco[J]. CHINESE TOBACCO SCIENCE, 2024, 45(1): 54-62. DOI: 10.13496/j.issn.1007-5119.2024.01.008

    酚氧化酶处理对白肋烟化学成分和烟叶品质的影响

    Effect of Phenol Oxidase Treatment on Chemical Composition and Leaf Quality of Burley Tobacco

    • 摘要: 为探索酚氧化酶处理在提升白肋烟烟叶品质方面的可行性,以未经醇化的湖北恩施白肋烟片烟为研究材料,将酚氧化酶施用于烟叶表面,研究了其在不同温度、酶液施加量及处理时间下对白肋烟烟叶内在化学组分及品质的影响。结果表明,酚氧化酶能够促进白肋烟中多种化学组分的改变,包括降低烟叶中特有亚硝胺(TSNAs)类和酚类物质含量,并促进酯类物质的积累。此外,酚氧化酶对烟叶中总糖及还原糖、总植物碱及酮类物质的作用效果受到处理条件的显著影响。在50 ℃、20%酶液添加量下施用酚氧化酶24 h后,烟叶中的TSNAs、酚类致香物质和酮类致香物质含量分别降低20.04%、27.08%和21.29%,还原糖、总糖、酯类致香物质含量分别增加44.92%、29.02%和11.27%,白肋烟的香气质、香气量、杂气、浓度、刺激性等多个指标分值提升1分以上,烟叶品质得到改善。综上,酚氧化酶施用于未经醇化的白肋烟叶,能够快速改善烟叶内在化学组分和提升烟叶品质。

       

      Abstract: In order to explore the feasibility of phenol oxidase treatment on improving the quality of burley tobacco leaves, phenol oxidase was applied to the surface of un-aged burley tobacco produced in Enshi City of Hubei Province, and its effects on the chemical composition and quality of burley tobacco leaves at different catalytic temperatures, dosages and catalytic time were studied. The results showed that phenol oxidase could promote the change of various chemical components in burley tobacco, including reducing the contents of tobacco-specific nitrosamines (TSNAs) and phenolic substances, and promoting the accumulation of esters. In addition, the effect of phenol oxidase on total sugars and reducing sugars, total phytobases and ketones in tobacco leaves was significantly affected by the catalytic conditions. After 24 hours of treatment using 20% phenol oxidase at 50 ℃, the contents of TSNAs, phenolic aroma compounds, and ketone aroma compounds in tobacco leaves decreased by 20.04%, 27.08% and 21.29%, respectively. The contents of reducing sugar, total sugar and ester aroma compounds increased by 44.92%, 29.02% and 11.27%, respectively. The scores of multiple indicators such as aroma quality, aroma amount, offensive odor, concentration, and irritation of burley tobacco increased by more than 1 point, indicating that the quality of tobacco leaves was improved. The results show that the application of phenol oxidase to un-aged burley tobacco leaves can quickly coordinate their intrinsic chemical components and improve their quality.

       

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