Abstract: For further details physiological specialization of Ralstonia solanacearum strains isolated from Hubei Province, 48 R.solanacearum strains of tobacco were analyzed using the phylotype classification schemes. Phylogenetic analysis of endoglucanase gene(egl) and DNA mismatch repair protein gene(mutS) sequences showed that all isolates belonged to phylotypeⅠ, including 3 different sequevars 15, 17 and 44. 19 strains were belonged to sequevar 17 and 26 strains were belonged to sequevar 44, Only 3 strains were identified as sequevar 15. R.solanacearum strains of tobacco from Hubei Province had genentic differentiation of sequevar level.