Abstract: In order to explore the microbial strains with the ability to alleviate the acidification of tobacco planting soil, a combination of biomorphology and molecular biology, pot experiment and field experiment were used to screen and identify high-yielding γ-PGA strains and study their improvement effects on acidified tobacco planting soil. The results showed that:(1) Aglutamate-independent γ-PGA producing strain Bacillus paralicheniformis 285-3 was screened from the arid soil of the Loess Plateau. (2) Strain 285-3 effectively alleviated soil acidification caused by root exudates and compound fertilizers, and increased soil pH by more than 0.3 units on average. (3) The combination of strain 285-3 and organic fertilizer significantly reduced the exchangeable acidity of acidified tobacco planting soil, and the control effect of tobacco bacterial wilt reached 47.05%, while improved the yield and quality of tobacco. In conclusion, Bacillus paralicheniformis 285-3 has a good improvement effect on acidified tobacco plating soil, and can control disease and improve yield and quality of tobacco when combined with organic fertilizer.